Organoid Models

Brain organoids are iPSC-derived models that recapitulate key features of human brain development and function. Multiple neural cell types self-organize into tissue-like structures, making it possible to study cellular interactions and region-specific biology in a controlled, human-relevant in vitro system.

Nocteva generates both whole-brain (cerebral) and region-specific organoids, a precise platform to investigate disease mechanisms, characterize cellular dysfunction, and evaluate therapeutic interventions where they matter most.

Midbrain

Ventral midbrain

Day 20 · BF

Day 50 · IF

FOXA2THDAPI

Cortex

Forebrain cortical

Day 20 · BF

Day 50 · IF

CTIP2SOX2RELN

Hippocampus

Dorsal hippocampal

Day 20 · BF

Day 75 · IF

PROX1SOX2

Retinal organoids recapitulate the key features of the human retina, including the presence and organization of all major retinal cell types. Derived from iPSCs, they provide a human-specific platform to study retinal development, disease mechanisms, and therapeutic interventions where animal models and 2D cultures fall short.

Nocteva has optimized retinal organoid generation for robust reproducibility, consistent cellular composition, and low batch-to-batch variability, with standardized differentiation protocols and rigorous quality control.

Photoreceptors & interneurons

Distinct cone and rod photoreceptor populations alongside amacrine interneurons

ARR3 — cone photoreceptors NRL — rod photoreceptors AP2α — amacrine cells Nuclei

Layered retinal circuitry

Rod photoreceptors, bipolar interneurons, and ganglion cells in native-like organization

RHO — rod photoreceptors CHX10 — bipolar cells & progenitors RBPMS — ganglion cells Nuclei

Resolved cellular organization

At high magnification, distinct photoreceptor populations and interneuron processes are clearly resolved within an ordered outer layer — direct evidence of the structural fidelity and cell-type diversity Nocteva's retinal organoids achieve.

RHO — rod photoreceptors CHX10 — bipolar cells & progenitors RBPMS — ganglion cells Nuclei

Nocteva's cardiac platforms span 2D cardiomyocyte monolayers to 3D cardiac organoids — human iPSC-derived models that recapitulate cardiac tissue composition, contractile activity, and electrophysiology for disease modeling and therapeutic evaluation.

Cardiac organoid generation is optimized and standardized for high reproducibility, consistent tissue composition, and robust functional performance.

TNNT2 — cardiomyocytesDAPI — nuclei

Live imaging — spontaneous beating

Brightfield — uniform monolayer morphology

iPSC-derived cardiomyocytes form spontaneously contracting 2D monolayers — a simple, scalable platform for cardiotoxicity screening and functional assays.

TNNT2 — cardiomyocytesCD31 — endothelial cellsDAPI — nuclei

Calcium imaging — live signaling during contraction

Brightfield — uniform, well-defined organoids

Live imaging captures rhythmic, spontaneous beating in real time — a direct functional readout of cardiac tissue health, drug response, and electrophysiological activity.

Nocteva builds a connected suite of human iPSC-derived neuromuscular models, from neuromuscular, skeletal muscle, and spinal cord organoids to functional spinal motor neurons, providing physiologically relevant platforms to study motor-neuron and muscle biology, disease, and therapeutic response.

Spinal cord organoids reproduce the cellular diversity and organization of the developing human spinal cord, including populations of motor neurons — a platform for studying motor circuits, spinal development, and motor-neuron disease.

Skeletal muscle organoids

Contractile human muscle tissue

Multinucleated muscle fibers, tissue organization, and contractile function.

TITIN — sarcomeric marker

Neuromuscular organoids

Functional neuromuscular junctions

Motor neurons, muscle cells, and terminal Schwann cells self-organize into functional neuromuscular junctions.

TUJ1 — motor neurons Fast myHC — muscle cells GFAP / S100b — Schwann cells αBTX — AChR clusters

iPSC-derived spinal motor neurons form dense, interconnected networks and mature into cholinergic neurons — enabling the study of motor-neuron biology and diseases such as ALS and spinal muscular atrophy.

Mature neuronal networks

Neurons interconnect and form spherical structures with extensive dendritic arbors

MAP2 — mature neurons & dendrites

Cholinergic identity

ChAT⁺ motor neurons — the enzyme that synthesizes acetylcholine for muscle activation

ChAT — cholinergic motor neurons Actin — cytoskeleton DAPI — nuclei

Lung organoids are iPSC-derived models that recapitulate key features of human airway and alveolar tissue, including epithelial organization and cell-type diversity, providing a human-relevant platform for studying lung development, disease, and therapeutic response.

Nocteva's apical-out lung organoids present the epithelial surface outward, mimicking the in vivo interface bacteria encounter — making them particularly suited for modeling bacterial infection and testing antibiotics.

BF

Live morphology — lung organoids in culture

IF

Overview — epithelial organization across organoids

IF

Apical-out organoid — ciliated surface facing outward

Patient-derived tumor organoids, including high-grade glioma and glioblastoma (GBM), are generated from patient tumor material and preserve the cellular heterogeneity, invasive behavior, and molecular signatures of the original tumor.

Nocteva establishes and characterizes tumor models for mechanistic studies, therapy testing, and biomarker discovery, with matched molecular profiling available through our multi-omics services.

Day 26 · BF

Live morphology — glioblastoma organoid (GBO) in culture

IF

Tumor cell populations — EGFR⁺ tumor cells and SOX2⁺ progenitor-like cells

EGFR — tumor cells SOX2 — progenitor-like cells